50 BP DNA STEP LADDER, FOR DNA ELECTROP&

Description_x000D_ General description_x000D_ Sigma’s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting._x000D_ Application_x000D_ Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels._x000D_ Components_x000D_ Sigma’s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA._x000D_ Other Notes_x000D_ Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands)._x000D_ For optimal resolution, the recommended agarose gel concentration is 2.0%.